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KMID : 0903519690110010049
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1969 Volume.11 No. 1 p.49 ~ p.53
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Studies on the Glucose Isomerizing Enzyme
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Seu J. H.
Kim C. K.
Ki W. K.
Rhee In-Koo
Kwon T. J.
Woo D. L.
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Abstract
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Some properties of glucose isomerizing enzyme which produced by the strain K-17 in xylose containing nutrient broth medium were investigated. The optimal pH for enzyme reaction was indicated about 7.2 and optimal temperature was about 75℃. The same optimal temperature was indicated by both cell free extract and acetone dried cells using as enzyme. The glucose isomerizing enzyme from strain K-17 was not inhibited by the high concentration of substrate even in a saturated glucose solution, but most enzyme was inactivated by the heat treatment at 80℃. The maximum fructose forming ratio from glucose was about 50 percents.
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